3 H ouabain binding studies indicated that Na(^+)+K(^+) exchange 'pump' turnover was adequate to account for substantial entry and Na^ exit across the basal membrane. The electrochemicalgradient across the apical membrane suggests that exit from the cell must involve an active process with CI following passively. Data from ion substitution experiments and treatment with furosemide and bumetanide suggest that CI entry across the basalmembrane may be via cotransport with Na^ and/or K^.
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